| 出品公司: | Promega |
|---|---|
| 载体名称: | PinPoint Xa-2 |
| 质粒类型: | 大肠杆菌表达载体；蛋白纯化 |
| 表达水平: | 高 |
| 启动子: | -- |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 3332 bp |
| 5' 测序引物及序列: | Sp6 Rev |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | Ampicillin |
| 筛选标记: | -- |
| 备注: | -- |
| 产品目录号: | V2051 |
| 稳定性: | 瞬表达 |
| 组成型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS PinPoint Xa-2 3332 bp DNA SYN
DEFINITION PinPoint Xa-2
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..3332
/organism="PinPoint Xa-2"
/mol_type="other DNA"
misc_feature 1..386
/label="biotin_Pinpoint_Xa-1"
CDS 1..489
/label="ORF frame 1"
/translation="MKLKVTVNGTAYDVDVDVDKSHENPMGTILFGGGTGGAPAPAAG
GAGAGKAGEGEIPAPLAGTVSKILVKEGDTVKAGQTVLVLEAMKMETEINAPTDGKVE
KVLVKERDAVQGGQGLIKIGDLELIEGRESFSWDPVPISDLPGRPRSGSIVSPKSYVY
DT*"
misc_feature complement(452..469)
/label="Sp6_primer"
/translation="MKLKVTVNGTAYDVDVDVDKSHENPMGTILFGGGTGGAPAPAAG
GAGAGKAGEGEIPAPLAGTVSKILVKEGDTVKAGQTVLVLEAMKMETEINAPTDGKVE
KVLVKERDAVQGGQGLIKIGDLELIEGRESFSWDPVPISDLPGRPRSGSIVSPKSYVY
DT*"
misc_feature 669..691
/label="pGEX_3_primer"
/translation="MKLKVTVNGTAYDVDVDVDKSHENPMGTILFGGGTGGAPAPAAG
GAGAGKAGEGEIPAPLAGTVSKILVKEGDTVKAGQTVLVLEAMKMETEINAPTDGKVE
KVLVKERDAVQGGQGLIKIGDLELIEGRESFSWDPVPISDLPGRPRSGSIVSPKSYVY
DT*"
promoter 850..878
/label="AmpR_promoter"
/translation="MKLKVTVNGTAYDVDVDVDKSHENPMGTILFGGGTGGAPAPAAG
GAGAGKAGEGEIPAPLAGTVSKILVKEGDTVKAGQTVLVLEAMKMETEINAPTDGKVE
KVLVKERDAVQGGQGLIKIGDLELIEGRESFSWDPVPISDLPGRPRSGSIVSPKSYVY
DT*"
gene 920..1780
/label="Ampicillin"
/gene="Ampicillin"
/translation="MKLKVTVNGTAYDVDVDVDKSHENPMGTILFGGGTGGAPAPAAG
GAGAGKAGEGEIPAPLAGTVSKILVKEGDTVKAGQTVLVLEAMKMETEINAPTDGKVE
KVLVKERDAVQGGQGLIKIGDLELIEGRESFSWDPVPISDLPGRPRSGSIVSPKSYVY
DT*"
CDS 920..1780
/label="ORF frame 2"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
rep_origin 1935..2554
/label="pBR322_origin"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter 2797..2815
/label="T7_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature 2939..2958
/label="pBRrevBam_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter 3210..3238
/label="tac_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature 3250..3272
/label="M13_pUC_rev_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 atgaaactga aggtaacagt caacggcact gcgtatgacg ttgacgttga cgtcgacaag
61 tcacacgaaa acccgatggg caccatcctg ttcggcggcg gcaccggcgg cgcgccggca
121 ccggcagcag gtggcgcagg cgccggtaag gccggagagg gcgagattcc cgctccgctg
181 gccggcaccg tctccaagat cctcgtgaag gagggtgaca cggtcaaggc tggtcagacc
241 gtgctcgttc tcgaggccat gaagatggag accgagatca acgctcccac cgacggcaag
301 gtcgagaagg tcctggtcaa ggagcgtgac gcggtgcagg gcggtcaggg tctcatcaag
361 atcggggatc tcgagctcat cgaaggtcgc gaaagcttca gctgggatcc ggtaccgata
421 tcagatctcc cggggcggcc gcgatctggt tctatagtgt cacctaaatc gtatgtgtat
481 gatacataag gttatgtatt aattgtagcc gcgttctaac gacaatatgt ccatatggtg
541 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
601 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
661 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
721 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
781 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
841 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
901 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
961 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
1021 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
1081 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
1141 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
1201 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
1261 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
1321 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
1381 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
1441 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
1501 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
1561 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
1621 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
1681 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
1741 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
1801 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
1861 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
1921 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
1981 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
2041 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
2101 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
2161 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
2221 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
2281 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
2341 gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
2401 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
2461 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
2521 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
2581 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
2641 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
2701 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
2761 gattcattaa tgcaggttaa cctggcttat cgaaattaat acgactcact atagggagac
2821 cggcctcgag cagcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca
2881 tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg
2941 tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga
3001 tgcgtccggc gtagaggatc gatccgggct tatcgactgc acggtgcacc aatgcttctg
3061 gcgtcaggca gccatcggaa gctgtggtat ggctgtgcag gtcgtaaatc actgcataat
3121 tcgtgtcgct caaggcgcac tcccgttctg gataatgttt tttgcgccga catcataacg
3181 gttctggcaa atattctgaa atgagctgtt gacaattaat catcggctcg tataatgtgt
3241 ggaattgtga gcggataaca atttcacaca ggaaacagaa ttcccagctt ggctgcagaa
3301 ccattccatt cgttgatccg ggagtaactc ac
//
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇：PinPoint Xa-1
- 下一篇：PinPoint Xa-3
